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Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis.

Identifieur interne : 000E42 ( Main/Exploration ); précédent : 000E41; suivant : 000E43

Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis.

Auteurs : Karolina Kucharska [Pologne] ; Rafał Łukajtis [Pologne] ; Edyta Słupek [Pologne] ; Hubert Cie Li Ski [Pologne] ; Piotr Rybarczyk [Pologne] ; Marian Kami Ski [Pologne]

Source :

RBID : pubmed:30463326

Descripteurs français

English descriptors

Abstract

The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products. Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied. Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification. The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal. Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions. The research was carried out using the Box-Behnken design. Additionally, the possibility of the re-use of both alkaline as well as enzymatic reagents was investigated. Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).

DOI: 10.3390/molecules23113029
PubMed: 30463326
PubMed Central: PMC6278490


Affiliations:


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Le document en format XML

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<term>Enzymes (metabolism)</term>
<term>Ethanolamine (chemistry)</term>
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<term>Lignine (composition chimique)</term>
<term>Populus (composition chimique)</term>
<term>Saccharose (composition chimique)</term>
<term>Techniques de culture cellulaire en batch (MeSH)</term>
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<div type="abstract" xml:lang="en">The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products. Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied. Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification. The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal. Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions. The research was carried out using the Box-Behnken design. Additionally, the possibility of the re-use of both alkaline as well as enzymatic reagents was investigated. Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by
<i>Enterobacter aerogenes ATCC 13048</i>
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